The ClinicianThe ClinicianКлиницист1818-83382412-8775Publishing House ABV Press16110.17650/1818-8338-2014-2-29-32ORIGINAL INVESTIGATIONSОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕIMMUNOHISTOCHEMICAL ANALYSIS OF CASPASE-3 ACTIVITY IN LIVER BIOPSIES OF PATIENTS WITH MONO AND MIXED INFECTIONSИММУНОГИСТОХИМИЧЕСКИЙ АНАЛИЗ АКТИВНОСТИ КАСПАЗЫ-3 В БИОПСИЯХ ПЕЧЕНИ ПАЦИЕНТОВ ПРИ МОНО- И СМЕШАННЫХ ИНФЕКЦИЯХTokinI. I.ТокинИ. И.
Russian Federation
ivan.i.tokin@rambler.ruTokinI. B.ТокинИ. Б.
Russian Federation
SologubT. V.СологубТ. В.
Russian Federation
FilimonovaG. F.ФилимоноваГ. Ф.
Russian Federation
HussarP.ХуссарП.
Russian Federation
Research Institute of Influenza, Ministry of Health of Russia, 15/17 Professor Popov St., St. Petersburg, 97376, Russia I.I. Mechnikov North-Western State Medical University, Ministry of Health of Russia, 47 Piskarevskiy proyezd, St. Petersburg, 195067, RussiaФГБУ «Научно-исследовательский институт гриппа» Минздрава России, Россия, 197376, Санкт-Петербург, ул. профессора Попова, 15/17 ГБОУ ВПО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Минздрава России, Россия, 195067, Санкт-Петербург, Пискаревский проезд, 47St. Petersburg State University,7 University Quay, St. Petersburg, 199034, RussiaФГБОУ ВПО «Санкт-Петербургский государственный университет», Россия, 199034, Санкт-Петербург, Университетская набережная, 7Research Institute of Influenza, Ministry of Health of Russia, 15/17 Professor Popov St., St. Petersburg, 97376, RussiaФГБУ «Научно-исследовательский институт гриппа» Минздрава России, Россия, 197376, Санкт-Петербург, ул. профессора Попова, 15/17I.I. Mechnikov North-Western State Medical University, Ministry of Health of Russia, 47 Piskarevskiy proyezd, St. Petersburg, 195067, Russia St. Petersburg State University,7 University Quay, St. Petersburg, 199034, RussiaГБОУ ВПО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Минздрава России, Россия, 195067, Санкт-Петербург, Пискаревский проезд, 47 ФГБОУ ВПО «Санкт-Петербургский государственный университет», Россия, 199034, Санкт-Петербург, Университетская набережная, 7Institute of Anatomy, Tartu University, 18 Yulikooli St., Tartu, 50090, EstoniaИнститут анатомии Тартуского университета, Эстония, 50090, Тарту, Юликооли, 18150420148229323001201530012015Copyright ©; 2014, Tokin I.I., Tokin I.B., Sologub T.V., Filimonova G.F., Hussar P.Copyright ©; 2014, Токин И.И., Токин И.Б., Сологуб Т.В., Филимонова Г.Ф., Хуссар П.2014Tokin I.I., Tokin I.B., Sologub T.V., Filimonova G.F., Hussar P.Токин И.И., Токин И.Б., Сологуб Т.В., Филимонова Г.Ф., Хуссар П.https://creativecommons.org/licenses/by/4.0https://klinitsist.abvpress.ru/Klin/article/view/161

Objective: to study the activity of proapoptotic signal protein caspase-3 for determination of peculiarities of apoptosis regulation under liver chronic diseases.

Subjects and methods. The immunohistochemical analysis of caspase-3 activity in 5 liver biopsies of the patients with mono infection of chronic hepatitis B and 5 liver biopsies of the patients with mixed infection of tuberculosis, chronic hepatitis C and human immunodeficiency virus was fulfilled. Morphological and morphometric analysis of serial microphotographs was performed using an image analysis system (microscope Leica DM 2500, digital camera Leica DFC320 R2 and a computer).

Results. The activity of caspase-3 as dark brown granularity was revealed in all tis-sue components of liver (hepatocytes, epithelium of bile ducts, endotheliocytes, Kupffer cells of sinusoids, in compositions of lymphohistiocyte infiltrations). The maximal activity was discovered in hepatocytes nuclei. The expression of caspase-3 was significantly higher in liver biopsies of the patients with mixed infection. It is typical that the immunoreactive hepatocytes had not any morphological marks of apoptosis.

Conclusion. The caspase-3 expression of proapoptotic signal protein caspase-3 may serve as an early marker of liver damage including the possibilities of apoptosis development.

apoptosisbiopsycaspase-3viral hepatitistuberculosishuman immunodeficiency virusапоптозбиопсиякаспаза-3вирусный гепатиттуберкулезвирус иммунодефицита человека1. Shi Y. Mechanisms of caspase activation and inhibition during apoptosis. Mol Cell 2002;9(3):459–70.Shi Y. Mechanisms of caspase activation and inhibition during apoptosis. Mol Cell 2002;9(3):459–70.2. Bantel H., Ruck P., Schultze-Osthoff K. In situ monitoring of caspase activation in hepatobiliary diseases. Cell Death Differ 2000;7(5):504–5.Bantel H., Ruck P., Schultze-Osthoff K. In situ monitoring of caspase activation in hepatobiliary diseases. Cell Death Differ 2000;7(5):504–5.3. Bantel H., Lugering A., Poremba C. et al. Caspase activation correlates with the degree of inflammatory liver injury in chronic hepatitis C virus infection. Hepatology 2001;34(4 Pt 1):758–67.Bantel H., Lugering A., Poremba C. et al. Caspase activation correlates with the degree of inflammatory liver injury in chronic hepatitis C virus infection. Hepatology 2001;34(4 Pt 1):758–67.4. Bantel H., Ruck P., Gregor M., Schulze-Osthoff K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur J Cell Biol 2001;80(3):230–9.Bantel H., Ruck P., Gregor M., Schulze-Osthoff K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur J Cell Biol 2001;80(3):230–9.5. Knodell R.G., Ishak K.G., Black W.C. et al. Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis. Hepatology 1981;1(5): 431–5.Knodell R.G., Ishak K.G., Black W.C. et al. Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis. Hepatology 1981;1(5): 431–5.6. Ischak K., Baptista A., Bianchi L. et al. Histological grading and staging of chronic hepatitis. J Hepatol 1995;22(6):696–9.Ischak K., Baptista A., Bianchi L. et al. Histological grading and staging of chronic hepatitis. J Hepatol 1995;22(6):696–9.7. Tokin I.B., Tokin I.I., Filimonova G.F. Computer image analysis of liver biopsy specimens in patients with heroin abuse and coinfection (tuberculosis, HCV, HIV). In: N. Tagaya (ed.). Liver biopsy – indications, procedures, results. 2012. Pp. 235–56.Tokin I.B., Tokin I.I., Filimonova G.F. Computer image analysis of liver biopsy specimens in patients with heroin abuse and coinfection (tuberculosis, HCV, HIV). In: N. Tagaya (ed.). Liver biopsy – indications, procedures, results. 2012. Pp. 235–56.8. McPartland J.L., Guzail M.A., Kendall C.H., Pringle J.H. Apoptosis in chronic viral hepatitis parallels histological activity: an immunohistochemical investigation using anti-activated caspase-3 and M30 cytodeath antibody. Int J Exp Pathol 2005;86(1): 19–24.McPartland J.L., Guzail M.A., Kendall C.H., Pringle J.H. Apoptosis in chronic viral hepatitis parallels histological activity: an immunohistochemical investigation using anti-activated caspase-3 and M30 cytodeath antibody. Int J Exp Pathol 2005;86(1): 19–24.9. Piekarska A,. Kubiak R., Omulecka A. et al. Expression of tumor necrosis factor-related apoptosis inducing ligand and caspase-3 in relation to grade of inflammation and stage of fibrosis in chronic hepatitis C. Histopathology 2007;51(5):597–604.Piekarska A,. Kubiak R., Omulecka A. et al. Expression of tumor necrosis factor-related apoptosis inducing ligand and caspase-3 in relation to grade of inflammation and stage of fibrosis in chronic hepatitis C. Histopathology 2007;51(5):597–604.10. Salama H., Medhat I., El-Makhzangy H. et al. Detection of caspase-3 activity in sera of egyptian patients with chronic HCV and normal ALT level. Med J Cairo Univ 2009;77(4):237–42.Salama H., Medhat I., El-Makhzangy H. et al. Detection of caspase-3 activity in sera of egyptian patients with chronic HCV and normal ALT level. Med J Cairo Univ 2009;77(4):237–42.11. Tokin I.В., Hussar P., Filimonova G. et al. Features of the liver apoptosis in patients with chronic hepatitis C viral infection (HCV). The 7th international Cell Death Society Symposium “Targeting cell death pathways for human diseases”. Shanghai, China, 2008. 127 p.Tokin I.В., Hussar P., Filimonova G. et al. Features of the liver apoptosis in patients with chronic hepatitis C viral infection (HCV). The 7th international Cell Death Society Symposium “Targeting cell death pathways for human diseases”. Shanghai, China, 2008. 127 p.12. Tokin I.B., Hussar P., Filimonova G. et al. Features of liver apoptosis in chronic hepatitis C virus infection. Papers on Anthropology 2009;18:361–71.Tokin I.B., Hussar P., Filimonova G. et al. Features of liver apoptosis in chronic hepatitis C virus infection. Papers on Anthropology 2009;18:361–71.13. Буеверов А.О. Апоптоз и вирусные гепатиты. Вирусные гепатиты: достижения и перспективы 2001;1(11):12–4. [Buyeverov A.O. Apoptosis and viral hepatites. Viral hepatites: аchievements and outlooks 2001;1(11):12–4. (In Russ.)].Буеверов А.О. Апоптоз и вирусные гепатиты. Вирусные гепатиты: достижения и перспективы 2001;1(11):12–4. [Buyeverov A.O. Apoptosis and viral hepatites. Viral hepatites: аchievements and outlooks 2001;1(11):12–4. (In Russ.)].